Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes
Identifieur interne : 001002 ( Main/Exploration ); précédent : 001001; suivant : 001003Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes
Auteurs : Aline Escalon [France] ; Stéphanie Javegny [France] ; Christian Vernière [France] ; Laurent D. Noël [France] ; Karine Vital [France] ; Stéphane Poussier [France] ; Ahmed Hajri [France] ; Tristan Boureau [France] ; Olivier Pruvost [France] ; Matthieu Arlat [France] ; Lionel Gagnevin [France]Source :
- Molecular Plant Pathology [ 1464-6722 ] ; 2013-06.
Abstract
The mechanisms determining the host range of Xanthomonas are still undeciphered, despite much interest in their potential roles in the evolution and emergence of plant pathogenic bacteria. Xanthomonas citri pv. citri (Xci) is an interesting model of host specialization because of its pathogenic variants: pathotype A strains infect a wide range of Rutaceous species, whereas pathotype A*/AW strains have a host range restricted to Mexican lime (Citrus aurantifolia) and alemow (Citrus macrophylla). Based on a collection of 55 strains representative of Xci worldwide diversity assessed by amplified fragment length polymorphism (AFLP), we investigated the distribution of type III effectors (T3Es) in relation to host range. We examined the presence of 66 T3Es from xanthomonads in Xci and identified a repertoire of 28 effectors, 26 of which were shared by all Xci strains, whereas two (xopAG and xopC1) were present only in some A*/AW strains. We found that xopAG (=avrGf1) was present in all AW strains, but also in three A* strains genetically distant from AW, and that all xopAG‐containing strains induced the hypersensitive response (HR) on grapefruit and sweet orange. The analysis of xopAD and xopAG suggested horizontal transfer between X. citri pv. bilvae, another citrus pathogen, and some Xci strains. A strains were genetically less diverse, induced identical phenotypic responses and possessed indistinguishable T3E repertoires. Conversely, A*/AW strains exhibited a wider genetic diversity in which clades correlated with geographical origin and T3E repertoire, but not with pathogenicity, according to T3E deletion experiments. Our data outline the importance of taking into account the heterogeneity of Xci A*/AW strains when analysing the mechanisms of host specialization.
Url:
DOI: 10.1111/mpp.12019
Affiliations:
- France
- La Réunion, Midi-Pyrénées, Occitanie (région administrative), Pays de la Loire, Région Bretagne
- Beaucouzé, Castanet‐Tolosan, Le Rheu, Saint‐Pierre, Toulouse
- Université Toulouse III - Paul Sabatier, Université d'Angers
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Xanthomonas citri pv. citri (Xci) is an interesting model of host specialization because of its pathogenic variants: pathotype A strains infect a wide range of Rutaceous species, whereas pathotype A*/AW strains have a host range restricted to Mexican lime (Citrus aurantifolia) and alemow (Citrus macrophylla). Based on a collection of 55 strains representative of Xci worldwide diversity assessed by amplified fragment length polymorphism (AFLP), we investigated the distribution of type III effectors (T3Es) in relation to host range. We examined the presence of 66 T3Es from xanthomonads in Xci and identified a repertoire of 28 effectors, 26 of which were shared by all Xci strains, whereas two (xopAG and xopC1) were present only in some A*/AW strains. We found that xopAG (=avrGf1) was present in all AW strains, but also in three A* strains genetically distant from AW, and that all xopAG‐containing strains induced the hypersensitive response (HR) on grapefruit and sweet orange. The analysis of xopAD and xopAG suggested horizontal transfer between X. citri pv. bilvae, another citrus pathogen, and some Xci strains. A strains were genetically less diverse, induced identical phenotypic responses and possessed indistinguishable T3E repertoires. Conversely, A*/AW strains exhibited a wider genetic diversity in which clades correlated with geographical origin and T3E repertoire, but not with pathogenicity, according to T3E deletion experiments. Our data outline the importance of taking into account the heterogeneity of Xci A*/AW strains when analysing the mechanisms of host specialization.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>La Réunion</li><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li><li>Pays de la Loire</li><li>Région Bretagne</li></region><settlement><li>Beaucouzé</li><li>Castanet‐Tolosan</li><li>Le Rheu</li><li>Saint‐Pierre</li><li>Toulouse</li></settlement><orgName><li>Université Toulouse III - Paul Sabatier</li><li>Université d'Angers</li></orgName></list><tree><country name="France"><region name="La Réunion"><name sortKey="Escalon, Aline" sort="Escalon, Aline" uniqKey="Escalon A" first="Aline" last="Escalon">Aline Escalon</name></region><name sortKey="Arlat, Matthieu" sort="Arlat, Matthieu" uniqKey="Arlat M" first="Matthieu" last="Arlat">Matthieu Arlat</name><name sortKey="Arlat, Matthieu" sort="Arlat, Matthieu" uniqKey="Arlat M" first="Matthieu" last="Arlat">Matthieu Arlat</name><name sortKey="Boureau, Tristan" sort="Boureau, Tristan" uniqKey="Boureau T" first="Tristan" last="Boureau">Tristan Boureau</name><name sortKey="Gagnevin, Lionel" sort="Gagnevin, Lionel" uniqKey="Gagnevin L" first="Lionel" last="Gagnevin">Lionel Gagnevin</name><name sortKey="Hajri, Ahmed" sort="Hajri, Ahmed" uniqKey="Hajri A" first="Ahmed" last="Hajri">Ahmed Hajri</name><name sortKey="Hajri, Ahmed" sort="Hajri, Ahmed" uniqKey="Hajri A" first="Ahmed" last="Hajri">Ahmed Hajri</name><name sortKey="Javegny, Stephanie" sort="Javegny, Stephanie" uniqKey="Javegny S" first="Stéphanie" last="Javegny">Stéphanie Javegny</name><name sortKey="Noel, Laurent D" sort="Noel, Laurent D" uniqKey="Noel L" first="Laurent D." last="Noël">Laurent D. Noël</name><name sortKey="Noel, Laurent D" sort="Noel, Laurent D" uniqKey="Noel L" first="Laurent D." last="Noël">Laurent D. Noël</name><name sortKey="Poussier, Stephane" sort="Poussier, Stephane" uniqKey="Poussier S" first="Stéphane" last="Poussier">Stéphane Poussier</name><name sortKey="Pruvost, Olivier" sort="Pruvost, Olivier" uniqKey="Pruvost O" first="Olivier" last="Pruvost">Olivier Pruvost</name><name sortKey="Verniere, Christian" sort="Verniere, Christian" uniqKey="Verniere C" first="Christian" last="Vernière">Christian Vernière</name><name sortKey="Vital, Karine" sort="Vital, Karine" uniqKey="Vital K" first="Karine" last="Vital">Karine Vital</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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